Experimental model for establishment of hypoxia in 75 cm 2 culture flasks 1 Sidney Mamoru Keira
نویسندگان
چکیده
In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2. In cell culture, it is possible to submit cells to hypoxic enviroment. The present study reports an alternative method at low cost for the establishment of hypoxic environment in cell culture flasks.
منابع مشابه
Experimental model for fibroblast culture
The use of cell culture methods in Plastic Surgery opened a new horizon in the research of cellular mechanisms of proliferation and biosynthesis functions. Several types of cells have been investigated in the cutaneous compartment. Keratinocytes and fibroblasts have been studied aiming the possibility of developing biomaterial for skin substitution. The present study describes the standardizati...
متن کاملMesangial cells derived from rat glomeruli produce an erythropoiesis stimulating factor in cell culture.
Erythropoietin (Ep) is a glycoprotein that stimulates the production of red blood cells. In response to hypoxia, the plasma level of Ep increases up to 2 or 3 orders of magnitude [ 11. The increase is absent if nephrectomized animals are exposed to hypoxia [2,3]. This indicates that the kidney is essential for the biosynthesis of Ep. Within the kidney, Ep production has been associated with the...
متن کاملigh-Density Culture of Human Islets on Top of Silicone ubber Membranes
Islet culture has emerged as a standard practice prior to clinical transplantation. However, culturing large numbers of islets requires low islet density (number of islets per unit surface area) and, consequently, 20 to 30 flasks per pancreas in order to avoid hypoxia-induced death (HID). There is a need for a simple, practical, small-footprint culture vessel that will accommodate aseptic maint...
متن کاملAn effective, low-cost method for achieving and maintaining hypoxia during cell culture studies.
Here we report a simple new method for exposing cells to normoxic and hypoxic conditions using vacuum bags, normally employed for food storage, to establish and maintain low oxygen levels in vitro. Vacuum bags were gassed with a mixture containing specified levels of oxygen, then sealed, creating a hypoxic microenvironment for cells cultured in flasks placed therein. Oxygen levels in the gas mi...
متن کاملEstablishment of a retinal hypoxia organ culture model
Hypoxia plays an important role in several retinal diseases, especially in central retinal artery occlusion (CRAO). Although CRAO has been known for over a hundred years, no cure or sufficient treatment is available. Potential therapies are being evaluated in several in vivo models or primary cultures. However, in vivo models or primary cultures are very time-consuming, expensive, and furthermo...
متن کامل